Abstract: Micro-arc oxidation(MAO) and hydrothermal synthesis(HS) technologies were used to prepare bio-film on the surface of titanium alloy.Surface morphologies and formation procedures of the films at various stages were observed by scanning electron microscope,and element and phase compositions of biological membrane layer were analyzed by energy dispersive spectroscopy and X-ray diffractometer.The cohesion between films and substrate materials was measured by scratching test,and the corrosion resistance of samples in Hank's simulated body fluid was probed into.MAO-HS samples and titanium alloy substrate samples were implanted into animal subcutaneous tissue after washing and sterilization.After 52 days of implanting,the samples were taken out and observed.The experimental results show that the cohesion between the films and substrates has a higher binding force of up to 26 N or so,and the corrosion resistance of MAO-HS samples is better than titanium alloy substrate specimen.Large tissues clamber over the surface of MAO-HS group,and the membrane layer contains a certain proportion of carbon and kalium element while there is no tissue growth found on the surface of titanium alloy substrates.Compared with the titanium alloy substrate material,MAO-HS samples shows more excellent histocompatibility as implants.